Figure 4
From: Direct transfection and activation of human cutaneous dendritic cells
Most of LC migrated form the epidermis in response to gene gun transfection by the time that EGFP expression can be detected in nonmigratory epidermal cells. (a) Population of resident LC (HLA-DR+, red) in an epidermal sheet immediately after gene delivery (100% of LC at time 0). (b) Shows the population of HLA-DR+ LC remaining in the epidermis, of the same skin sample but 24 h after transfection. (c) The same epidermal sheet as in b shows the expression of EGFP in nonmigratory epidermal cells (mostly keratinocytes) 24 h after transfection (×200) (scale bar, 10 μm).
