Figure 8
From: Direct transfection and activation of human cutaneous dendritic cells
LC isolated from pMART-1 transfected human epidermis present antigen to MART-1/Melan A27–35 specific CTLs. CD1a+ cells obtained from human skin after delivery of pCI-MART1, or an identical quantity of pBackbone, were co-cultured with an HLA-A2-restricted CTL. The capability of transfected LC to induce specific T cell activation assessed by measuring the levels of INF-γ released by the CTL is shown. Additional controls included incubation of CD1a+ cells, CTL alone or transfected LC incubated with the blocking HLA-A2 mAb BB7.1. The pCI-MART1 transfected LC stimulated significantly higher levels of INF-γ release than pCI backbone transfected controls (P = 0.0001). INF-γ release was significantly reduced by the addition of BB7.1 mAb (P = 0.0001). Means ± 1 s.d. from five independent studies are shown.
