Figure 1

Sequence information for SARS-CoV used for siRNA design. (a) Leader sequence information from different transcripts of the SARS-CoV. The transcripts of SARS-CoV from the infected Vero E6 cells were 5′-RACE and RT-PCR amplified and sequenced. The identical Leader sequences were boxed. The start codons (ATG) for different genes are underlined with italic letters. The targeted sequence in the Leader sequence of SARS-CoV is underlined. The one-step RT-PCR reaction condition was 35 cycles of denaturation (94°C, 30 s), annealing (51°C, 45 s) and extension (72°C: S gene, 1 min; E gene, 25 s; M gene, 45 s; N gene, 1 min 20 s). (b) Schematic of vector for generating siRNA. pBS/U6 promoter, sequence generating siRNA hairpin and transcriptional terminal signal are shown as indicated. (c) Diagram of predicted structure of siRNA from pBS/U6/L-RNAi. UUCAAGAGA was used to generate the hairpin loop, indicated as a cycle.