Figure 3

Inhibition of reporter gene expression by siRNA. The reporter constructs as indicated were cotransfected with the pBS/U6/L-RNAi (lanes 1–4) or pBS/U6/S-RNAi (lanes 5–8) into 293T cells. Total RNA was isolated for performance of RT-PCR using the primers as indicated. The comparison of the effect of siRNA should be between lanes 1 and 5, 2 and 6, 3 and 7, and 4 and 8. β-actin was used as an internal control. The PCR reaction conditions were 25 cycles of denaturation (94°C, 30 s), annealing (50°C, 1 min) and extension (72°C, 1 min).