Figure 4

Fluorescence microscopy observation of reporter gene expression in transfected cells. 293T cells were cotransfected with the reporter constructs and the pBS/U6/L-RNAi (A) or pBS/U6/S-RNAi (B) vector. The reporter constructs used are a, b and c, and j, k and l: pEFBos/GFP and pDsRed1.1/RFP; d, e and f, and m, n and o: PEFBos/L-GFP and pDsRed1.1/RFP; g, h and i, and p, q and r: pDsRed1.1/L-RFP and PEFBos/GFP. The same field was observed by red color (excitation, 510–560 nm; emission, 590 nm) and green color (excitation, 450–490 nm; emission, 520 nm) and the two color pictures were merged in the right panel. Note that pBS/U6/L-RNAi significantly inhibited reporter gene expression (e and g).