Abstract
The Shaker gene of Drosophila melanogaster encodes a potassium-selective ion channel, the 'A' channel, or one of its subunits. A single Shaker messenger RNA species suffices to direct the synthesis of functional A channels in Xenopus oocytes. Physiological characteristics of the A currents induced by two different mRNA species are compared.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hille, B. Ionic Channels of Excitable Membranes (Sinauer, Sunderland, 1984).
Shuster, M. J., Camardo, J. S., Siegelbaum, S. A. & Kandel, E. R. Nature 313, 392–395 (1985).
Farley, J. & Auerbach, S. Nature 319, 220–223 (1986).
Kamb, A., Iverson, L. E. & Tanouye, M. A. Cell 50, 405–413 (1987).
Papazian, D. M., Schwarz, T. L., Tempel, B. L., Jan, Y. N. & Jan, L. Y. Science 237, 749–753 (1987).
Tempel, B. L., Papazian, D. M., Schwarz, T. L., Jan, Y. N. & Jan, L. Y. Science 237, 770–775 (1987).
Baumann, A. et al. EMBO J. 6, 3419–3430 (1987).
Salkoff, L. & Wyman, R. Nature 293, 228–230 (1981).
Wu, C.-F. & Haugland, F. J. Neurosci. 10, 2626–2640 (1985).
Timpe, L. C. & Jan, L. Y. J. Neurosci. 7, 1307–1317 (1987).
Solc, C. K., Zagotta, W. N. & Aldrich, R. W. Science 236, 1094–1098 (1987).
Connor, J. A. & Stevens, C. F. J. Physiol. 213, 21–30 (1971).
Neher, E. J. gen. Physiol. 58, 36–53 (1971).
Noda, M. et al. Nature 312, 121–127 (1984).
Tanabe, T. et al. Nature 328, 313–318 (1987).
Schwarz, T. L., Tempel, B. L., Papazian, D. M., Jan, Y. N. & Jan, L. Y. Nature 331, 137–142 (1988).
Gurdon, J. B. & Wickens, M. P. Meth. Enzym. 101, 370–386 (1983).
Salkoff, L. & Wyman, R. J. Physiol. 337, 687–709 (1983).
Thompson, S. H. J. Physiol. 265, 465–488 (1977).
Melton, W. A. et al. Nucleic Acids Res. 12, 7035–7057 (1984).
Contreras, R., Cheroutre, H. & Fiers, W. et al. Analyt. Biochem. 113, 185–187 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Timpe, L., Schwarz, T., Tempel, B. et al. Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes. Nature 331, 143–145 (1988). https://doi.org/10.1038/331143a0
Received:
Accepted:
Published:
Issue date:
DOI: https://doi.org/10.1038/331143a0
This article is cited by
-
35 years of channelling potassium ions
Nature (2022)
-
Hsp70 affects memory formation and behaviorally relevant gene expression in Drosophila melanogaster
Cell Stress and Chaperones (2021)
-
Operation of a homeostatic sleep switch
Nature (2016)
-
Spinocerebellar ataxia type 19/22 mutations alter heterocomplex Kv4.3 channel function and gating in a dominant manner
Cellular and Molecular Life Sciences (2015)
-
Energetic role of the paddle motif in voltage gating of Shaker K+ channels
Nature Structural & Molecular Biology (2013)
