Abstract
Adjuvant arthritis (AA) is a chronic disease inducible in rats by immunization with an antigen of Mycobacterium tuberculosis1. After the isolation of arthritogenic T-cell lines2 and clones3, it became possible to demonstrate that the critical M. tuberculosis antigen contained an epitope cross-reactive with a self-antigen in joint cartilage4–6. Like AA rats, patients suffering from rheumatoid arthritis demonstrated specific T-lymphocyte reactivity to the M. tuberculosis fraction containing the cross-reactive epitope7. To characterize the critical M. tuberculosis epitope we used AA T-cell clones to screen mycobacterial antigens expressed in Escherichia coli and genetically engineered truncated proteins and synthetic peptides. The AA T-cell clones recognized an epitope formed by the amino acids at positions 180–188 in the sequence of a Mycobacterium bovis BCG antigen8. Administration of this antigen to rats induced resistance to subsequent attempts to produce AA.
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Eden, W., Tholet, J., Zee, R. et al. Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis. Nature 331, 171–173 (1988). https://doi.org/10.1038/331171a0
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DOI: https://doi.org/10.1038/331171a0
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