Abstract
Pulsed field gel techniques have pushed the size limits for the separation of DNA fragments forward by several orders of magnitude. By orienting the electric field transverse to the gel, lane-to-lane distortion of DNA migration can be eliminated.
Enjoying our latest content?
Log in or create an account to continue
- Access the most recent journalism from Nature's award-winning team
- Explore the latest features & opinion covering groundbreaking research
or
References
Fangman, W.L. Nucleic Acids Res. 5, 653–665 (1978).
Schwartz, D.C. & Cantor, C.R. Cell 337, 67–75 (1984).
Carle, G.F. & Olson, M.V. Nucleic Acids Res. 12, 5647–5664 (1984).
Gardiner, K., Laas, W. & Patterson, D. Somatic Cell molec. Genet. 12, 185–195 (1986).
Schwartz, D.C. et al. Cold Spring Harb. Symp. quant. Biol. 47, 189–195 (1983).
Van der Ploeg, L.H.T. et al. Cell 37, 77–84 (1984).
Chu, G., Vollrath, D. & Davis, R.W. Science 234, 1582–1585 (1987).
McPeek, F.D., Coyle-Morris, J.F. & Gemmill, R.M. Analyt. Biochem. 156, 274–285 (1986).
Carle, G.F., Frank, M. & Olson, M.V. Science 232, 65–68 (1986).
Gemmill, R.M., Coyle Morris, J.F., McPeek, F.D., Ware-Uribe, L. & Hecht, F. Gen. analyt. Tech. 4, 119–131 (1987).
Umesono, K., Hiraoka, Y., Toda, T. & Yanagida, M. Curr. Genet. 7, 123–128 (1983).
Niwa, O., Matsumoto, T. & Yanagida, M. Molec. Gen. Genet. 203, 397–405 (1986).
Rights and permissions
About this article
Cite this article
Gardiner, K., Patterson, D. Transverse alternating electrophoresis. Nature 331, 371–372 (1988). https://doi.org/10.1038/331371a0
Issue date:
DOI: https://doi.org/10.1038/331371a0
This article is cited by
-
Analysis of the chromosomal DNA polymorphism of wine strains of Saccharomyces cerevisiae
Current Genetics (1992)
-
Polymorphic karyotypes in related Acremonium strains
Current Genetics (1991)
-
Low Frequency Restriction Enzymes in Pulsed Field Electrophoresis
Nature Biotechnology (1989)
-
TheIL-4 andIL-5 genes are closely linked and are part of a cytokine gene cluster on mouse chromosome 11
Somatic Cell and Molecular Genetics (1989)