Abstract
THE spatial organization of the Drosophila embryo depends on the activity of three axial pattern-forming systems. In addition to the anterior-posterior and dorsal-ventral systems that organize the segmented body plan1, a proximal-distal pattern-forming system is required to provide positional information for the developing limbs. The development of both the larval and adult limbs depends directly on the activity of the Distal-less gene2,3. Genetic analysis has shown that Distal-less functions as a developmental switch that is required to promote the development of limb structures above the evolutionary ground-state of body wall. Here we provide genetic evidence that indicates a graded requirement for Distal-less activity during limb development. Reduction of this activity has a global effect on pattern formation in the limb. The molecular structure of the Distal-less locus indicates that the gene encodes a homoeodomain-containing protein which is therefore likely to specify limb development through differential regulation of subordinate genes.
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Ingham, P. W. Nature 335, 25–34 (1988).
Cohen, S. M. & Jürgens, G. EMBO J., submitted.
Sunkel, C. E. & Whittle, J. R. S. Roux's Archs dev. Biol. 196, 124–132 (1987).
Campos-Ortega, J. & Hartenstein, V. The Embryonic Development of Drosophila melanogaster (Springer, Berlin, 1985).
Gehring, W. J. Science 236, 1245–1252 (1987).
Hall, M. N. & Johnson, A. Science 237, 1007–1012 (1987).
Balling, R., Deutsch, U. & Gruss, P. Cell 55, 531–535 (1988).
Barad, M., Jack, T., Chadwick, R. & McGinnis, W. EMBO J. 7, 2151–2161 (1988).
Mlodzik, M., Fjose, A. & Gehring, W. J. EMBO J. 7, 2569–2578 (1988).
Desplan, C., Theis, J. & O'Farrell, P. Nature 318, 630–635 (1985).
Hoey, T. & Levine, M. Nature 332, 858–861 (1988).
Driever, W. & Nüsslein-Volhard, C. Nature 337, 138–143 (1988).
Cho, K. W. Y. et al. EMBO J. 7, 2139–2149 (1988).
Müller, M. et al. EMBO J. 7, 4299–4304 (1988).
Beachy, P. A., Krasnow, M. A., Gavis, E. R. & Hogness, D. S. Cell 55, 1069–1081 (1988).
Hoey, T., Warrior, R., Manak, J. & Levine, M. Molec. cell. Biol. 8, 4598–4402 (1988).
Desplan, C., Theis, J. & O'Farrell, P. Cell 54, 1081–1090 (1988).
Oliver, G., Wright, C. V. E., Hardwicke, J. & DeRobertis, E. M. Cell 55, 1017–1024 (1988).
Savard, P., Gates, P. B. & Brockes, J. P. EMBO J. 7, 4275–4282 (1988).
Frohnhöfer, H. G. & Nüsslein-Volhard, C. Nature 324, 120–125 (1986).
Driever, W. & Nüsslein-Volhard, C. Cell 54, 83–93 (1988).
Driever, W. & Nüsslein-Volhard, C. Cell 54, 95–104 (1988).
Snodgrass, R. E. Principles of Insect Morphogenesis (McGraw-Hill, London, 1935).
Page, D. et al. Cell 51, 1091–1104 (1987).
Beveriey, S. M. & Wilson, A. C. J. molec. Evol. 21, 1–13 (1984).
Langer-Safer, P. R., Levine, M. & Ward, D. C. Proc. natn. Acad. Sci. U.S.A. 79, 4381–4385 (1982).
Otting, G. et al. EMBO J. 7, 4305–4309 (1988).
Pabo, C. O. & Sauer, R. T. A. Rev. Biochem. 53, 293–321 (1984).
Poole, S., Kauvar, L., Drees, B. & Kornberg, T. Cell 40, 37–43 (1985).
Fjose, A., McGinnis, W. & Gehring, W. J. Nature 313, 284–289 (1985).
Weinzierl, R., Axton, M., Ghysen, A. & Akam, M. Genes Dev. 1, 386–397 (1987).
Rushlow, C., Doyle, H., Hoey, T. & Levine, M. Genes Dev. 1, 1268–1297 (1987).
Saint, R., Kalionis, B., Lockett, T. J. & Elizur, A. Nature 334, 151–154 (1988).
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Cohen, S., Brönner, G., Küttner, F. et al. Distal-less encodes a homoeodomain protein required for limb development in Drosophila. Nature 338, 432–434 (1989). https://doi.org/10.1038/338432a0
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DOI: https://doi.org/10.1038/338432a0
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