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Association of a Ras-related protein with cytochrome b of human neutrophils

Nature volume 342, pages 198–200 (1989)Cite this article

Abstract

ACTIVATION of the superoxide generating system in human neutrophils is thought to involve the interaction or assembly of cytochrome b with other cytosolic and membrane proteins. We have now co-isolated by conventional purification procedures a protein of relative molecular mass 22,000 with cytochrome b. This Ras-related protein is not a fragment of either of the subunits of cytochrome b, and its primary structure, as determined by the sequencing of its complementary DNA, is identical to that predicted from a recently cloned ras- related gene, rap 1 (also termed Krev-1). Immunoaffinity purification on anti-cytochrome and anti-Ras immunoaffinity matrices indicates an association between cytochrome b and the Ras-related protein. The association of a Ras-related GTP-binding protein with cytochrome b of human neutrophils could indicate a role for such a protein in the transduction, regulation or structure of the superoxide generating system.

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Author notes

  1. Charles A. Parkos

    Present address: Department of Pathology, Brigham & Women's Hospital, 75 Francis Street, Boston, Massachusetts, 02115, USA

Authors and Affiliations

  1. Department of Chemistry, Montana State University, Bozeman, Montana, 59717, USA

    Mark T. Quinn & Algirdas J. Jesaitis

  2. Research Institute of Scripps Clinic, 10666 North Torrey Pines Road, La Jolla, California, 92037, USA

    Charles A. Parkos & Leslie Walker

  3. Division of Hematology-Oncology, Children's Hospital and Dana-Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Stuart H. Orkin & Mary C. Dinauer

  4. Howard Hughes Medical Institute, Children's Hospital, Boston, Massachusetts, 02115, USA

    Stuart H. Orkin

Authors
  1. Mark T. Quinn
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  2. Charles A. Parkos
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  3. Leslie Walker
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  4. Stuart H. Orkin
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  5. Mary C. Dinauer
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  6. Algirdas J. Jesaitis
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Quinn, M., Parkos, C., Walker, L. et al. Association of a Ras-related protein with cytochrome b of human neutrophils. Nature 342, 198–200 (1989). https://doi.org/10.1038/342198a0

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