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In vitro replication through nucleosomes without histone displacement

Nature volume 343, pages 719–726 (1990)Cite this article

Abstract

A well-characterized set of proteins encoded by bacteriophage T4 replicates DNA in vitro and generates replication forks that can pass nucleo-somes. The histone octamers remain associated with newly replicated DNA even in the presence of excess DNA competitor, and intact nucleosomes re-form on the two daughter DNA helices. It is concluded that nucleosomes are designed to open up transiently to allow the passage of a replication fork without histone displacement.

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Author notes

  1. Catherine Bonne-Andrea

    Present address: Centre de Biochimie, Universite de Nice, Pare Valrose, 06034, Nice, France

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143-0448, USA

    Catherine Bonne-Andrea, Mei Lie Wong & Bruce M. Alberts

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  1. Catherine Bonne-Andrea
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  2. Mei Lie Wong
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  3. Bruce M. Alberts
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Bonne-Andrea, C., Wong, M. & Alberts, B. In vitro replication through nucleosomes without histone displacement. Nature 343, 719–726 (1990). https://doi.org/10.1038/343719a0

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