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A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein β-adaptin

Nature volume 349, pages 215–220 (1991)Cite this article

Abstract

Four high-molecular-weight proteins form the main subunits of the coat of Golgi-derived (non-clathrin) coated vesicles. One of these coat proteins, β-COP, is identical to a Golgi-associated protein of relative mass 110,000 (110K) that shares homology with the adaptin proteins of clathrin-coated vesicles. This connection, and the comparable molecular weights of the coat proteins of Golgi-derived and clathrin-coated vesicles, indicates that they may be structurally related. The identification of β-COP as the 110K protein explains the blocking of secretion by the drug brefeldin A.

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References

  1. Jamieson, J. D. & Palade, G. E. J. Cell Biol. 34, 577–596 (1967).

    Article  CAS  Google Scholar 

  2. Palade, G. Science 189, 347–358 (1975).

    Article  ADS  CAS  Google Scholar 

  3. Rothman, J. E., Miller, R. L. & Urbani, L. J. J. Cell Biol. 99, 260–261 (1984).

    Article  CAS  Google Scholar 

  4. Rothman, J. E., Urbani, L. J. & Brands, R. J. Cell Biol. 99, 248–259 (1984).

    Article  CAS  Google Scholar 

  5. Pearse, B. M. F. J. molec. Biol. 97, 93–98 (1975).

    Article  CAS  Google Scholar 

  6. Pearse, B. M. F. & Bretscher, M. S. A. Rev. Biochem. 50, 85–101 (1981).

    Article  CAS  Google Scholar 

  7. Orci, L., Ravazzola, M., Amherdt, M., Louvard, D. & Perrelet, A. Proc. natn. Acad. Sci. U.S.A. 82, 5385–5389 (1985).

    Article  ADS  CAS  Google Scholar 

  8. Griffiths, G., Pfeiffer, S., Simons, K. & Matin, K. J. Cell Biol. 101, 949–964 (1985).

    Article  CAS  Google Scholar 

  9. Orci, L., Glick, B. S. & Rothman, J. E. Cell 46, 171–84 (1986).

    Article  CAS  Google Scholar 

  10. Wieland, F. T., Gleason, M. L., Serafini, T. A. & Rothman, J. E. Cell 50, 289–300 (1987).

    Article  CAS  Google Scholar 

  11. Karrenbauer, A. et al. Cell 63, 259–267 (1990).

    Article  CAS  Google Scholar 

  12. Morris, S. A., Ahle, S. & Ungewickell, E. Curr. Opin. Cell. Biol. 1, 684–690 (1989).

    Article  CAS  Google Scholar 

  13. Keen, J. H. A. Rev. Biochem. 59, 415–438 (1990).

    Article  CAS  Google Scholar 

  14. Balch, W. E., Dunphy, W. G., Braell, W. A. & Rothman, J. E. Cell 39, 405–416 (1984).

    Article  CAS  Google Scholar 

  15. Balch, W. E., Fries, E., Dunphy, W. H., Urbani, L. J. & Rothman, J. E. Meth. Enzym. 98, 37–47 (1983).

    Article  CAS  Google Scholar 

  16. Balch, W. E. & Rothman, J. E. Arch. Biochem. Biophys. 240, 413–425 (1985).

    Article  CAS  Google Scholar 

  17. Balch, W. E., Glick, B. S. & Rothman, J. E. Cell 39, 525–536 (1984).

    Article  CAS  Google Scholar 

  18. Orci, L., Malhotra, V., Amherdt, M., Serafini, T. & Rothman, J. E. Cell 56, 357–368 (1989).

    Article  CAS  Google Scholar 

  19. Melançon, P. et al. Cell 51, 1053–1062 (1987).

    Article  Google Scholar 

  20. Malhotra, V., Serafini, T., Orci, L., Shepherd, J. C. & Rothman, J. E. Cell 58, 329–336 (1989).

    Article  CAS  Google Scholar 

  21. Allan, V. J. & Kreis, T. E. J. Cell Biol. 103, 2229–2239 (1986).

    Article  CAS  Google Scholar 

  22. Donaldson, J. G., Uppincott-Schwartz, G. S., Bloom, G. S., Kreis, T. E. & Klausner, R. D. J. Cell Biol. 111, 2295–2306 (1990).

    Article  CAS  Google Scholar 

  23. Misumi, Y. et al. J. biol. Chem. 261, 11398–11403 (1986).

    CAS  Google Scholar 

  24. Oda, K. et al. FEBS Lett. 214, 135–138 (1987).

    Article  CAS  Google Scholar 

  25. Waters, M. G., Serafini, T. & Rothman, J. E. Nature, 349, 248–251 (1991).

    Article  ADS  CAS  Google Scholar 

  26. Schook, W., Puszkin, S., Bloom, W., Ores, C. & Kochwa, S. Proc. natn. Acad. Sci. U.S.A. 76, 116–120 (1979).

    Article  ADS  CAS  Google Scholar 

  27. Keen, J. H., Willingham, M. C. & Pastan, I. H. Cell 16, 303–312 (1979).

    Article  CAS  Google Scholar 

  28. Pearse, B. M. F. Proc. natn. Acad. Sci. U.S.A. 79, 451–455 (1982).

    Article  ADS  CAS  Google Scholar 

  29. Zaremba, S. & Keen, J. H. J. Cell Biol. 97, 1339–1347 (1983).

    Article  CAS  Google Scholar 

  30. Pearse, B. M. F. & Robinson, M. S. EMBO J. 3, 1951–1957 (1984).

    Article  CAS  Google Scholar 

  31. Keen, J. H. J. Cell Biol. 105, 1989–1998 (1987).

    Article  CAS  Google Scholar 

  32. Glick, B. S. & Rothman, J. E. Nature 326, 309–312 (1987).

    Article  ADS  CAS  Google Scholar 

  33. Block, M. R., Glick, B. S., Wilcox, C. A., Wieland, F. T. & Rothman, J. E. Proc. natn. Acad. Sci. U.S.A. 85, 7852–7856 (1988).

    Article  ADS  CAS  Google Scholar 

  34. Wilson, D. W. et al. Nature 339, 355–359 (1989).

    Article  ADS  CAS  Google Scholar 

  35. Malhotra, V., Orci, L., Glick, B. S., Block, M. R. & Rothman, J. E. Cell 54, 221–227 (1988).

    Article  CAS  Google Scholar 

  36. Robinson, M. S. J. Cell Biol. 111, 2319–2326 (1990).

    Article  CAS  Google Scholar 

  37. Ahle, S., Mann, A., Eichelsbacker, U. & Ungewickell, E. EMBO J. 7, 919–929 (1988).

    Article  CAS  Google Scholar 

  38. Ahle, S. & Ungewickell, E. J. biol. Chem. 264, 20089–20093 (1989).

    CAS  PubMed  Google Scholar 

  39. Pearse, B. M. EMBO J. 7, 3331–3336 (1988).

    Article  CAS  Google Scholar 

  40. Glickman, J. N., Conibear, E. & Pearse, B. M. EMBO J. 8, 1041–1047 (1989).

    Article  CAS  Google Scholar 

  41. Härri, E., Loeffler, W., Sigg, H. P., Stähelin, H. & Tamm, H. Helv. Chim. Acta. 46, 1235–1243 (1963).

    Article  Google Scholar 

  42. Fujiwara, T., Oda, K., Yokota, S., Takatsuki, A. & Ikehara, Y. J. biol. Chem. 263, 18545–18552 (1988).

    CAS  PubMed  Google Scholar 

  43. Lippincott-Schwartz, J., Yuan, L. C., Bonifacino, J. S. & Klausner, R. D. Cell 56, 801–813 (1989).

    Article  CAS  Google Scholar 

  44. Doms, R. W., Russ, G. & Yewdell, J. W. J. Cell Biol. 109, 61–72 (1989).

    Article  CAS  Google Scholar 

  45. Lippincott-Schwartz, J., Donaldson, J. G., Schweizer, A., Berger, E. G. & Hauri, H.-P. Cell 60, 821–836 (1990).

    Article  CAS  Google Scholar 

  46. Munro, S. & Pelham, H. R. B. Cell 48, 899–907 (1987).

    Article  CAS  Google Scholar 

  47. Pelham, H. R. B. EMBO J. 7, 913–918 (1988).

    Article  CAS  Google Scholar 

  48. Bensadoun, A. & Weinstein, D. Analyt. Biochem. 70, 241–250 (1976).

    Article  CAS  Google Scholar 

  49. Peterson, G. L. Analyt Biochem. 83, 346–356 (1977).

    Article  CAS  Google Scholar 

  50. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  51. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

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Author notes

  1. James E. Rothman: Address from 1 June 1991

Authors and Affiliations

  1. Department of Biochemistry, Stanford University, Stanford, California, 94305, USA

    Tito Serafini

  2. Department of Molecular Biology, Princeton University, Princeton, New Jersey, 08544, USA

    Tito Serafini & James E. Rothman

  3. Institut für Biochemie I, Universität Heidelberg, D-6900, Heidelberg, Germany

    Gudrun Stenbeck, Andreas Brecht & Felix T. Wieland

  4. Genzentrum, Max Planck Institut für Biochemie, D-8033, Martinsried bei München, Germany

    Friedrich Lottspeich

  5. Département de Morphologic, Universite dé Genève, 1211, Genève, 4, Switzerland

    Lelio Orel

  6. Program in Molecular Cell Biology, Rockefeller Research Laboratory, Sloan-Kettering Institute, 1275 York Avenue, New York, New York, 10021, USA

    James E. Rothman

Authors
  1. Tito Serafini
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  2. Gudrun Stenbeck
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  6. James E. Rothman
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  7. Felix T. Wieland
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Serafini, T., Stenbeck, G., Brecht, A. et al. A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein β-adaptin. Nature 349, 215–220 (1991). https://doi.org/10.1038/349215a0

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