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I-POU: a POU-domain protein that inhibits neuron-specific gene activation

Nature volume 350, pages 577–584 (1991)Cite this article

Abstract

A novel, structurally distinct POU-domain protein has been identified that inhibits activation by another positive POU-domain regulator of neuron-specific transcription units. Two Drosophila POU-domain proteins, I-POU and Cfl-a, are coexpressed in overlapping subsets of neurons during development. Because I-POU lacks two basic residues in the N terminus of its homeodomain, it cannot bind DNA, but it does form a stable heterodimeric complex with Cfl-a, preventing Cfl-a from binding to DNA recognition elements and from transactivating the dopa-decarboxylase gene. The inhibition by I-POU provides a potential strategy by which the activation of genes in development is controlled by a homeodomain-containing protein that does not bind DNA.

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Authors and Affiliations

  1. Eukaryotic Regulatory Biology Program, University of California, San Diego

    Maurice N. Treacy, Xi He & Michael G. Rosenfeld

  2. School of Medicine, 9500 Gilman Drive, La Jolla, California, 92093-0648, USA

    Maurice N. Treacy, Xi He & Michael G. Rosenfeld

  3. Howard Hughes Medical Institute, University of California, San Diego

    Maurice N. Treacy & Michael G. Rosenfeld

  4. School of Medicine, 9500 Gilman Drive, La Jolla, California, 92093-0648, USA

    Maurice N. Treacy & Michael G. Rosenfeld

  5. Department of Biology, University of California, San Diego

    Xi He

  6. School of Medicine, 9500 Gilman Drive, La Jolla, California, 92093-0648, USA

    Xi He

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  1. Maurice N. Treacy
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  2. Xi He
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  3. Michael G. Rosenfeld
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Treacy, M., He, X. & Rosenfeld, M. I-POU: a POU-domain protein that inhibits neuron-specific gene activation. Nature 350, 577–584 (1991). https://doi.org/10.1038/350577a0

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