Table 1. Ago mutations in human cancer cell lines

From: Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines

CANCER CELL LINE

NUCLEOTIDE CHANGE AND POSITION

EFFECT ON PROTEIN

FREQUENCY IN CONTROLS

OV1063 (Ovarian)

G to T at nt 118 (Homozygous)

Premature truncation (E40Ter)

ND

MDAH 2774 (Ovarian)

G to A at splice acceptor (Heterozygous)

Frameshift (261Ter)

0/160

SK-OV3 (Ovarian)

G to T at nt 1274 (Heterozygous)

Missense (R425L)

0/185

CCRF-CEM (ALL)

C to T at nt 1153 (Heterozygous)

Missense (R385C)

0/185

  1. Nucleotide positions refer to the cDNA of the b-form where position 1 corresponds to the A in the start codon. The mutation in MDAH 2774 results from an AG to AA change in the splice acceptor site of exon 5 resulting in the use of an adventitious splice acceptor site 37 nucleotides downstream. The resulting deletion in the mRNA shifts the reading frame at codon 208 and adds 41 incorrect amino acids after this codon prior to termination.
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