Table 4: Primers used for the amplification of ADAMTS13 exons and intron/exon boundaries
From: Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura
Exon | Forward Primer Sequence | Reverse Primer Sequence | Annealing Temp. |
|---|---|---|---|
1 | 5’-ccc tga act gca acc atc tt-3’ | 5’-caa acc cca aag ctg atg ta-3’ | 561 |
2 | 5’-tcg gtc tcc cca agt gtt ag-3’ | 5’-aac agg gtt gac agc agc tt-3’ | 561 |
3 | 5’-tct aga acc atc gcc ctc tg-3’ | 5’-ccg agc cat tct acc tga gt-3’ | 561 |
4 | 5’-gcc tct cca gct ctt cac ac-3’ | 5’-gca ttc tgt gat cca tgc tg-3’ | 561 |
5-6 | 5’-acg ggc tag tca tag ggt tg-3’ | 5’-tac aag gac cca ctg ctt gc-3’ | 561 |
7 | Unavailable | unavailable | Â |
8 | 5’-CTT CCA AAC GCT TCC ATC CT-3’ | 5’-CCC TCC CAG GAC TAG CTA CA-3’ | 562 |
9 | 5’-TCT GGG AGG GAC AGT TAA GG-3’ | 5’-TAC TGG TCC TGC CTC CTG AC-3’ | 561 |
10-11 | 5’-GGG ATC CCT ATG GGT GAG TT-3’ | 5’-CCT GGT GTG AAC CAC AGA TG-3’ | 561 |
12 | 5’-GCA CTT TTG TCA CCC CAG TT-3’ | 5’-CCA GAG CCT GAA CCA CTT TG-3’ | 562 |
13-14 | 5’-CCC AGA TGC AAA GGA TGA AG-3’ | 5’-ATC CAG GGC TGA GTG AGT GT-3’ | 561 |
15 | 5’-TTT TTC CCG ACC AGC TAA GA-3’ | 5’-TCA GAA GTG AGG GCA TCT TG-3’ | 561 |
16 | 5’-CCG GGA AGG AGA GTC ACT G-3’ | 5’-CCC TGT AAG TGA CCG CTG A-3’ | 601 |
17-18 | 5’-gtg att gct tgc tga acg aa-3’ | 5’-cag tgt cct cac ctg cag aa-3’ | 561 |
19 | 5’-gaa cac ctg gag agg cta gg-3’ | 5’-act tac aac cgc cag gtg ac-3’ | 583 |
20 | 5’-GAA CCT GCT GGC TGA TGA AT-3’ | 5’-GGA TGG TGT TCT TGC TCT GG-3’ | 561 |
21 | 5’-CAC ACA CGC CAC TTC CTG-3’ | 5’-CCA CGT GTT CCC ATA TAG TCT G-3’ | 561 |
22 | 5’-CAC AGC TGG TAA GTG GCA GA-3’ | 5’-CAC AGC TGG TAA GTG GCA GA-3’ | 601 |
23 | 5’-TCC CAG CTT CCT GTC TCT TC-3’ | 5’-TCT CCT GAT TCA GCT TTC CAA-3’ | 601 |
24 | 5’-AGT ACA CGT GGG TGG AGA GG-3’ | 5’-CTT TCA GGG GAC ACG ATG AG-3’ | 561 |
25 | 5’-TTA ACT GCC TCC CAG CTT GT-3’ | 5’-CTT TGC CAG GGA GAA AGA GG-3’ | 563 |
26-27 | 5’-ACA GGG TCC ACC CCT ACC T-3’ | 5’-CCC AGT TCC TTC CAT CTC AG-3’ | 561 |
28 | 5’-TAT TGA CCA CAG TGC CAT GC-3’ | 5’-TGG TGA ATA TGT GGA GGA AGG-3’ | 561 |
29 | 5’-CCT CGG TTT TCT GGG TAG AG-3’ | 5’-CCA TCC TCG GAG TGG AAT C-3’ | 561 |
