Figure 1: Detection of HIV-1 sequences in the L70 plasma sample by RT-PCR.
From: An African HIV-1 sequence from 1959 and implications for the origin of the epidemic
The HIV-1 genome is shown at the top. ZR59 fragments (a, b, c and d) that were successfully amplified are shown in the middle, as are the primers used. Results of the successful RT-PCR are shown on the bottom. M, molecular marker; lane 1, positive plasma control; lane 2, negative plasma control; lane 3, reagent control (sterile water); lane 4, L70 plasma without reverse transcription before PCR; lane 5, L70 plasma; lane 6, serum from an infected patient identified in 1985; lane 7, serum from an infected patient (H.) identified in 1994. Each gel corresponds to the ZR59 fragment it is shown under. LTR, long terminal repeat.
