Abstract
RAT basophilic leukaemia cells, like mast cells from which they are derived, have surface Feɛ receptors that trigger secretion of inflammatory mediators when crosslinked. Both GIF-binding proteins and a rise in cytosolic calcium concentration ([Ca2+]i) are implicated in the secretory mechanism1–5. Here we use a video-imaging technique to report that transient rises in [Ca2+]i initiated in an individual cell can spread from cell to cell in a wave-like pattern by means of a secreted intermediate, in the absence of gap-junctional communication. We find that the leukaemia cells, peritoneal mast cells and mucosal mast cells have cell-surface P2-type purinergic receptors that can trigger similar [Ca2+]i transients. We provide evidence that ATP is rapidly released, and that it can amplify [Ca2+]i signals and initial secretory responses during antigen-stimulation of rat basophilic leukaemia cells.
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Osipchuk, Y., Cahalan, M. Cell-to-cell spread of calcium signals mediated by ATP receptors in mast cells. Nature 359, 241–244 (1992). https://doi.org/10.1038/359241a0
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DOI: https://doi.org/10.1038/359241a0
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