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DNA topoisomerase I is involved in both repression and activation of transcription

Nature volume 365, pages 227–232 (1993)Cite this article

Abstract

Reconstituted transcription reactions containing the seven general transcription factors, in addition to RNA polymerase II, respond poorly to transcriptional activators. Two factors, Dr2 and ACF, necessary for high levels of transcription in response to an activator have been identified. ACF can enhance basal and activated transcription. Dr2 represses basal transcrip-tion, but this can be overcome by transcriptional activators or TFIIA. Dr2 is human DNA topoiso-merase I. The DNA relaxation activity of topoisomerase I is dispensable for transcriptional repression. The effect of Dr2 is specific for TATA-box-containing promoters and is mediated by the TATA-binding protein.

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Authors and Affiliations

  1. Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey, 08854-5635, USA

    Alejandro Merino & Danny Reinberg

  2. Department of Microbiology, University of Washington, Seattle, Washington, 98195, USA

    Knut R. Madden & James J. Champoux

  3. Harvard Microchemistry Facility, Cambridge, Massachusetts, 02138, USA

    William S. Lane

Authors
  1. Alejandro Merino
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  2. Knut R. Madden
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  3. William S. Lane
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  4. James J. Champoux
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  5. Danny Reinberg
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Merino, A., Madden, K., Lane, W. et al. DNA topoisomerase I is involved in both repression and activation of transcription. Nature 365, 227–232 (1993). https://doi.org/10.1038/365227a0

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