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BUD2 encodes a GTPase-activating protein for Budl/Rsrl necessary for proper bud-site selection in yeast

Nature volume 365, pages 269–274 (1993)Cite this article

Abstract

CELLS of the budding yeast Saccharomyces cerevisiae bud at either axial or bipolar sites depending on their cell type1. Bud-site selec-tion directs both cell polarity and the cytoskeletal orientation dur-ing budding and is determined by at least five genes: BUD1/RSR1, BUD2, BUD3, BUD4 and BUD52—4. Mutants defective in BUD1, BUD2 or BUD5 choose bud sites randomly. Budl protein (Budlp) has sequence similarity to Ras2, a small GTP-binding protein, and Bud5p is similar to Cdc25p (refs 4, 5), a GDP—GTP exchange factor6. Here we report that Bud2p is a GTPase-activating protein (GAP) for Budlp with a sequence similar to the catalytic domain of rasGAPs, and that Bud2p purified from yeast stimulates GTP hydrolysis by Budlp. Chromosomal deletion of BUD2 causes a random budding pattern but no obvious growth defect. Overexpres-sion of BUD2 also causes a random budding pattern by wild-type cells11.

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  1. John Chant

    Present address: Department of Biochemistry & Molecular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

Authors and Affiliations

  1. Programs in Genetics and Cell Biology, Department of Biochemistry & Biophysics, University of California, San Francisco, San Francisco, California, 94143-0448, USA

    Hay-Oak Park, John Chant & Ira Herskowitz

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  1. Hay-Oak Park
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  2. John Chant
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  3. Ira Herskowitz
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Park, HO., Chant, J. & Herskowitz, I. BUD2 encodes a GTPase-activating protein for Budl/Rsrl necessary for proper bud-site selection in yeast. Nature 365, 269–274 (1993). https://doi.org/10.1038/365269a0

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