Messenger RNA (mRNA) sequences were amplified from whole blood prepared in guanidine thiocyanate (GuSCN) after capture of the mRNA with an affinity membrane.
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References
Sambrook, J., Fritsch, E. F. & Maniatis, T. in Molecular Cloning, 2nd edn (Cold Spring Harbor Laboratory, New York, 1989).
Chomzynski, P. & Sacci, N. Analyt. Biochem. 162, 156–159 (1987).
Thompson, J. & Gillespie, D. H. Analyt. Biochem. 163, 281–291 (1987).
Vogelstein, B. & Gillespie, D. H. Biochem. biophys. Res. Commun. 75, 1127–1132 (1977).
Casey, J. & Davidson, N. Nucleic Acids Res. 4, 1539–1552 (1977).
Saiki, R. K. et al. Science 239, 487 (1988).
Cuddy, K. K., Foley, B., Jaffe, J. & Gillespie, D. H. Nucleic Acids Res. 21, 2281 (1993).
Cuddy, K. K. et al. Edgwood Research Development and Engineering Center Symp., Aberdeen, Maryland, U.S.A. Nov. 17–19, 1993 (in the press).
Thompson, J. D., Brodsky, I. & Yunis, J. J. Blood 79, 1629–1635 (1992).
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Gillespie, D., Cuddy, K., Kolbe, T. et al. Dissolve and capture: a strategy for analysing mRNA in blood. Nature 367, 390–391 (1994). https://doi.org/10.1038/367390a0
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DOI: https://doi.org/10.1038/367390a0
