Figure 1
Transendothelial migration across an intact endothelial monolayer. (a) Identification of the monocytic cell population by CD45/CD14 staining. R1 represents the dual-stained population of interest. (b) Quantification of migrating monocytes in response to 12.5 nM MCP-1 for 90 min; R2 represents the migrating monocytes and R3 the control population of fluorescent beads. (c) Determination of the optimum concentration of MCP-1 for transendothelial migration. The figure shows representative data from two separate experiments with three replicates for each condition; the points represent mean values and the error bars show the standard error. (d) Chemotaxis in response to 12.5 nM MCP-1 in the basal chamber. The figure shows representative data from one of three separate experiments with three replicates for each condition; the points represent the mean values and the error bars show the standard error. (e) Chemotaxis assay comparing the results produced following the addition of 12.5 nM MCP-1 to either the basal or apical chambers. The figure shows representative data from one of four separate experiments with three replicates for each condition; the points represent the mean values and the error bars show the standard error. (f) Chemotaxis assay comparing the results produced following the addition of either 12.5 nM P8A or 12.5 nM wild-type MCP-1. The figure shows representative data from one of six separate experiments with three replicates for each condition; the points represent the mean values for this experiment; the error bars indicate the standard error.
