Figure 2
Asymmetric localization of MCP-1 following the addition of 12.5 nM MCP-1 to the subendothelial compartment. (a) Concentrations of MCP-1 in the upper experimental chamber. (b) Concentrations of MCP-1 in the lower experimental chamber. Mean data in (a) and (b) were derived from six separate experiments with three replicates for each condition; the error bars show the standard error. (c) P8A concentrations in the upper chamber of the trans-well system after basal application of the mutant protein for 90 min at 37°C. The representative data shown are from one of five separate experiments with three replicates for each condition; mean values are shown together with the standard error.
