Figure 15 | Laboratory Investigation

Figure 15

From: Different types of glomerulopathic light chains interact with mesangial cells using a common receptor but exhibit different intracellular trafficking patterns

Figure 15

(a, b) Crosslinking of G-LCs to HMCs membrane. SDS-PAGE transferred to PVDF membranes. C—Coomassie blue; D—Mass spectroscopy of shifted bands. Note the shifted band containing LC (normally present at about 25 kD) at approximately 300–325 kDa (a, b) and 450 kDa (resolved into three separate bands on Coomassie blue stain—C) representing the fragmented G-LC—MC complex. Mass spectroscopy matched filamin and talin, components of caveolin-1, as constituents of the shifted bands at 300—325 kDa. A weak match for kinase anchor protein was also detected in the band located at 450 kDa.

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