Figure 5
From: Antiapoptotic action of hypoxia-inducible factor-1α in human endothelial cells
Group comparisons of Q-PCR analyses of HIF-1α mRNA expression showing the effects of normoxia, A/R, and transfection with scrambled RNA duplexes on cultured HUVEC. Transfection with HIF-1α siRNA (but not scrambled RNA) duplexes prior to A/R inhibited HIF-1α mRNA expression. Results in each group category (other than group a) represent mean±s.e.m. of three experiments. (A) Normoxic cultures. (B) Cultures transfected with HIF-1α siRNA duplexes and subjected to A/R. (C) Cultures transfected with scrambled RNA duplexes and subjected to A/R. (D) Nontransfected cultures subjected to A/R. *Significantly different (P<0.05) compared with groups C and D.
