Figure 7
From: Canine malignant hemangiosarcoma as a model of primitive angiogenic endothelium
Aggregation of canine HSA cell lines into tubular structures. HSA cell lines were cultured under conditions of low calcium (0.1 mM EDTA) with FK506 (100 ng/ml) without endothelial growth supplements. Cells were examined microscopically after 4–6 h under phase contrast illumination. Panel a shows an example of DD-1 cells (× 100 magnification) and panel b shows an example of DAL-4 cells (× 320 magnification) to illustrate the active migration and aggregation of cells to form elongated structures. Panels c and d are photomicrographs of CHAD-G8.2 cells (× 100 magnification) illustrating a vessel-like structure and an end bud, respectively.
