Figure 5
From: Human heparanase nuclear localization and enzymatic activity
Nuclear colocalization of heparanase and heparan sulfate. Stable H-hpa-transfected U87 glioma cells (pooled population) were subjected to indirect immunofluorescence staining with (a) monoclonal anti-HS antibodies (mAb 3G10) followed by Cy2-conjugated goat anti-mouse antibody (green), and (b) polyclonal antiheparanase antibodies (733) followed by Cy3-conjugated goat anti-rabbit antibodies (red), as described in ‘Materials and methods’, (c and d) Colocalization of nuclear HS and heparanase (yellow, arrows). Immunofluorescent staining was analyzed by confocal microscopy. Images were obtained at an original objective magnification of × 60. There was no staining of cells exposed to Cy2- or Cy3-conjugated antibodies alone.
