Figure 1 | Laboratory Investigation

Figure 1

From: Short consensus probes with 3′-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas

Figure 1

Schematic diagrams of the probe/primer design used in the present study (a) and a comparison of consensus probe locations between ALL and B-NHL (b). (a), A consensus probe is designed for the VH gene segments with an ASO reverse primer designed complementary to the VH–DH or DH–JH junctional region and a patient-specific forward primer designed from the same strand for each germline. Each primer is indicated by an arrow and the probe by a bar. (b) In childhood ALL, consensus regions for probes are relatively wide because their sequences are all germline configuration. Consensus regions in B-NHL are narrow because of somatic hypermutations (indicated by vertical lines).

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