Figure 2
From: Analysis of the vitamin D system in basal cell carcinomas (BCCs)
Real-time PCR analysis (LightCycler) of VDR (a), 1α-OHase (b), 24-OHase (c), and 25-OHase (d) genes in BCCs and NS using target gene-specific hybridization probes as described in Materials and methods. The median of ratio target gene/GAPDH indicates the relative expression of target gene-specific mRNA, normalized for RNA loading. Note that ratios of VDR/GAPDH (BCCs: median: 16.54; NS: median: 0.00021), 1α-OHase/GAPDH (BCCs: median: 0.739; NS: median: 0.000803), and 24-OHase/GAPDH (BCCs: median: 0.585; NS: median: 0.000000365) gene expression were significantly increased in BCCs as compared to NS. In contrast, ratio of 25-OHase/GAPDH (BCCs: median: 0.167; NS: median: 0.0166) was not significantly altered in BCCs as compared to NS.
