Figure 2

Micrographs showing histological appearance of H&E stained sections of IR injured gastrocnemius muscle subjected to 70min warm ischaemia followed by either 24 h reperfusion (a–c) or 3 day reperfusion (d–f). Micrographs show normal littermate (a and d), W^f/W^f (b and e) and BMMC engrafted W^f/W^f (c and f). Note: similar appearance of skeletal muscle after 24 h, due to the failure of histological manifestations of necrosis at this early time point. At 3 days reperfusion, it is clearly evident that gastrocnemius muscle from normal littermate (d) and BMMC engrafted W^f/W^f (f) have more necrotic fibres (lighter pink colour) and less healthy fibres (darker pink colour as indicated by arrows) than the W^f/W^f muscle (e). n≥4 for all groups. Magnification × 200. Scale bar=100 μm.