Figure 4
From: Human neutrophils produce interferon gamma upon stimulation by interleukin-12
RT-PCR analysis of IFNγ mRNA expression in PMN. Total RNA was extracted from resting (PMN−) and activated PMN (LPS+IL-12+IL-15) (PMN+). After reverse transcription, PCR was carried out with specific pairs of primers designed for IFNγ and β-actin. Total lung tissue served as positive control (+). The figure indicates the size of amplification products relative to molecular weight standards run in parallel (MW) and the negative control (−) (PCR buffer without cDNA).
