Figure 5
From: Human neutrophils produce interferon gamma upon stimulation by interleukin-12
Western blot analysis of IFNγ expression by human PMN. Total homogenates of resting purified PMN or LPS+IL-12-treated PMN were added to 2 × Laemmli sample buffer, and proteins were resolved by SDS-PAGE. Proteins were immunoblotted after transfer to nitrocellulose membranes. Recombinant human IFNγ (rh IFNγ) served as positive control.
