Figure 6

Biological activity of neutrophil-derived IFNγ. PMN (10⁷/ml) (a) or PBMC (0.5 × 10⁶/ml) (b) were cultured in the presence of 100 ng/ml LPS, with or without PMN culture supernatants containing IFNγ (obtained in previous experiments). Recombinant human IFNγ (rhIFNγ 250 IU/ml) was used as a positive control. Each culture was done in the presence or absence of anti-IFNγ neutralizing antibody (1 μg/ml). Cell-free supernatants were harvested after 24 h of culture at 37°C in 5% CO₂, and were stored at −70°C until TNFα and IL-10 assays. ^*P<0.05 vs LPS.