Table 2 Effect of CHX on stimulus-induced IFNγ secretion by PMN

From: Human neutrophils produce interferon gamma upon stimulation by interleukin-12

 

Without CHX pretreatment

With CHX pretreatment

Control PMN

8±5

8±4

LPS+IL-12+IL-15

872±423

10±5*

  1. PMN (2 × 106/ml) were preincubated with or without CHX and then stimulated for 24 h at 37°C in the presence of LPS+IL-12+IL-15 or in medium alone. IFNγ was assayed in the cell-free supernatants. Results are expressed as the means±s.e.m. of three experiments.
  2. *P<0.05 as compared with cells incubated without CHX pretreatment.
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