Table 3 Effect of degranulating agents on IFNγ secretion by human PMN

From: Human neutrophils produce interferon gamma upon stimulation by interleukin-12

 

Cell-associated

Secreted

Control 4°C

33±17

8±5

Control 37°C

19±4

11±9

Cyt B+fMLP

8±5*

18±7

LPS+IL-12+IL-15

10±4*

20±7

  1. Secreted IFNγ was measured in cell lysates and supernatants of of PMN (107/ml) maintained at 4°C or incubated for 15 min at 37°C with or without inducers of degranulation. Results are expressed as the mean±s.e.m. of six independent experiments.
  2. *P<0.05 vs control PMN at 4°C.
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