Figure 2 | Laboratory Investigation

Figure 2

From: Identification of genes involved in VEGF-mediated vascular morphogenesis using embryonic stem cell-derived cystic embryoid bodies

Figure 2

Effect of exogenous VEGF or FGF-1 on vessel structures in VEGF-null CEB. Top panels: Fluorescent localization of PECAM-1-positive vessel structures in CEB formed from wt ES and VEGF-null ES cells. For rescue studies physiological doses of VEGF (10 ng/ml) or bFGF (1 ng/ml) were added to day 4 CEB, the time when high VEGF expression is first observed in the wt CEB. Bottom panels: Effect of VEGF (left) and bFGF (right) addition on vessel formation in VEGF-null CEB. VEGF rescues the formation of vascular cords and networks in VEGF-null CEB, whereas addition of bFGF promotes the proliferation of EC and enlargement of the endothelial clusters. Bars=100 μm.

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