Figure 3 | Laboratory Investigation

Figure 3

From: Identification of genes involved in VEGF-mediated vascular morphogenesis using embryonic stem cell-derived cystic embryoid bodies

Figure 3

Expression of VEGF and VEGFR2 receptor messages during CEB differentiation. (a) A Northern blot of total RNA isolated from differentiating CEB probed for VEGF expression. Lanes labeled ‘sparse’ and ‘dense’ contain total RNA isolated from sparse and dense cultures of undifferentiated ES cells, respectively. (b) Quantitation of VEGF mRNA levels (normalized to GAPDH levels) during CEB differentiation based on the results in (a). Expression of VEGF commenced on day 4 of CEB differentiation, and the levels of VEGF mRNA increased steadily from day 4 to day 9. (c) RT-PCR analysis of total RNA isolated from differentiating CEB for Flk-1 expression showing that the expression of VEGFR2 commenced on day 4 of CEB differentiation. Lane m, size marker. Lane RT(−), the negative control for the RT reactions in which no reverse transcriptase was added. Lane PCR(−), the negative control for the PCR reactions in which no DNA template was added. Lane PCR(+), the positive control for the PCR reaction, in which 5 ng of VEGFR2 plasmid DNA was used.

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