Figure 7 | Laboratory Investigation

Figure 7

From: Identification of genes involved in VEGF-mediated vascular morphogenesis using embryonic stem cell-derived cystic embryoid bodies

Figure 7

Expression of caveolin-1 and endomucin-1 protein in CEB formed from wt and VEGF-null ES cells. (a) Top panels: Double staining for caveolin-1 protein (green) and PECAM-1 protein (red) confirm that caveolin-1 is highly expressed by EC in day 16 wt CEB. Note vesicular nature of the caveolin-1 staining in wt EC. Bottom panels: PECAM-1 staining reveals the presence of EC clusters in VEGF-null CEB and very low levels of caveolin-1 staining. (b) Top panels: Double staining for endomucin-1 protein (green) and PECAM-1 protein (red) in wt CEB show that EC express high levels of endomucin-1. Also note the cell-surface nature of the endomucin-1 staining on wt EC. Bottom panels: The PECAM-1 positive EC cluster (red) in VEGF-null CEB have no detectable endomucin-1 protein staining.

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