Figure 2

Identification of SYT region required for interaction with mSin3A. Truncated SYT proteins used for yeast two-hybrid liquid culture assay are shown at left, and β-galactosidase activity is shown at right. A black stripe indicates a 31 amino-acid insertion in the SYT variant, SYT(+31), and the truncated SYT variant, SYT(+31)(1-BP). Arrows show break point. Error bars indicate the standard deviation obtained from three independent assays.