Figure 3 | Laboratory Investigation

Figure 3

From: SYT, a partner of SYT-SSX oncoprotein in synovial sarcomas, interacts with mSin3A, a component of histone deacetylase complex

Figure 3

Interaction of SYT-SSX proteins with mSin3A. Intensity of the interaction is shown as β-galactosidase activity by yeast two-hybrid liquid culture assay. SYT-SSX1 and SYT-SSX2 proteins show less interaction with mSin3A. When the C-terminal region (144–188 amino acids) was removed, the β-galactosidase activity was restored using SYT-SSX2T. SYT(+31)-SSX 1 and 2 were composed of the N-terminal 1–410 amino-acid region of SYT(+31) and the C-terminal 111–188 amino-acid region of SSX1 and 2, respectively. SYT-SSX2T was composed of the N-terminal region of SYT and the C-terminal 111–143 amino acid region of SSX2. SSX-DR is located on 155–188 amino acids region of SSX. Error bars indicate the standard deviation obtained from three independent assays.

Back to article page