Figure 5 | Laboratory Investigation

Figure 5

From: SYT, a partner of SYT-SSX oncoprotein in synovial sarcomas, interacts with mSin3A, a component of histone deacetylase complex

Figure 5

Pull-down assay for SYT and mSin3A. GST-SYT, GST-SYT-SSX1 and GST-truncated SYT (1-BP) were purified from transiently transfected HEK293T cells with glutathione–sepharose beads and incubated with 35S-methionine-labeled in vitro translated mSin3A. Specific binding of mSin3A to GST-SYT, GST-SYT-SSX1 and GST-SYT(1-BP) were detected (lanes 4–6, respectively). Translated lysate without mRNA and GST empty vector were used as negative controls (lanes 1 and 3, respectively). Input, sample representing total input (0.6%) for each experiment.

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