Figure 2

Western blot analysis of MTAP protein expression in colon carcinoma cell lines and HT29M3 cells. In five out of six colon carcinoma cell lines the expression of MTAP protein was almost as strong as in healthy liver that was used as a positive control with known high MTAP protein expression. In contrast, HT29M3 cells showed only minimal MTAP expression. Beta-actin staining was performed to ensure equal loading.