Table 2 RT-PCR primers for Toll-like receptors
GenBank accession | Forward | 5′–3′ | Exon | Reverse | 5′–3′ | Exon | Size |
|---|---|---|---|---|---|---|---|
TLR1 AL050262 | 5′-ATA-ACA-AAG-GTA-TAT-TGG-GCA-3′ | 174–194 | 3 | 5′-RS-TGT-TCT-TCA-GAT-CAT-CTT-GAT-3′ | 241–221 | 4 | 93 |
TLR2 U88878 | 5′-GTG-GGG-CTC-ATT-GTG-CCC-ATT-3′ | 61–81 | 2 | 5′-RS-CAT-CCA-CAA-AGT-ATG-TGG-CAT-3′ | 150–130 | 3 | 115 |
TLR3 U88879 | 5′-AAA-AGG-AAA-GGC-TAG-CAG-TCA-3′ | 41–61 | 1 | 5′-RS-AGC-ATC-CCA-AAG-GGC-AAA-AGG-3′ | 132–112 | 2 | 117 |
TLR4 NM003266 | 5′-AGT-TTC-CCA-GAA-CTG-CAG-GTG-3′ | 513–533 | 3 | 5′-RS-GCT-TAG-GCT-CTG-ATA-TGC-CCC-3′ | 593–573 | 4 | 106 |
TLR5 AF051151 | 5′-AGT-CCC-TTC-TGC-TAG-GAC-AAC-3′ | 676–696 | 5 | 5′-RS-AAG-GAA-TTC-CAA-ACA-CAG-GAC-3′ | 770–750 | 6 | 120 |
TLR6 AB020807 | 5′-CAA-AAG-ACC-TAC-CGC-TGA-AAA-3′ | 207–227 | 1 | 5′-RS-TGA-TAG-AAA-GCT-CAT-GTC-AGA-3′ | 295–275 | 1 | 114 |
TLR7 AF245702 | 5′-TTT-GGA-AGA-AGA-CTA-AAA-ATG-3′ | 116–136 | 2 | 5′-RS-AAG-GAG-TTT-GGA-AAT-TAG-GAT-3′ | 208–188 | 3 | 118 |
TLR8 AF245703 | 5′-TTC-TGC-GCT-GCT-GCA-AGT-TAC-3′ | 1–21 | 1 | 5′-RS-ACG-ACT-GAA-GGA-ACA-TGT-TTT-3′ | 73–53 | 2 | 98 |
TLR9 AF245704 | 5′-AAG-CCC-CTG-CCC-CCC-AGC-ATG-3′ | 127–147 | 1 | 5′-RS-CCA-GCA-TGA-TGG-CCT-GCA-CCA-3′ | 205–185 | 2 | 104 |
TLR10 AF296673 | 5′-ATT-ATG-CTT-CTC-CTC-TCT-GAG-3′ | 306–326 | 1 | 5′-RS-AGA-GCA-TTG-GCT-GAG-AAG-TCT-3′ | 396–376 | 1 | 116 |
