Figure 2 | Laboratory Investigation

Figure 2

From: Overexpression of fatty acid synthase in chemically and hormonally induced hepatocarcinogenesis of the rat

Figure 2

Quantitative RT-PCR (Light-Cycler) of FAS mRNA (left side) and the housekeeping gene β-actin (right side). For determining the amplification efficiency of the RT-PCR, serial dilutions of known concentrations of the plasmid probe of β-actin or serial dilutions of the cDNA of normal liver tissue (upper row) were simultaneously amplified with the mRNA of both, the laser-microdissected liver tissue of foci of altered hepatocytes (FAH) and unaltered liver tissue (control) of the same animal (bottom row). The example depicted in this diagram showed a 9.1-fold overexpression of FAS (for calculation method see Materials and methods).

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