Table 1 Sequences of primers, hybridization probes, annealing temperatures and lengths of PCR-products

From: Overexpression of fatty acid synthase in chemically and hormonally induced hepatocarcinogenesis of the rat

Rat-fatty acid synthase: NM 017332a, 281-bpb, annealing temperature: 65°C

FAS-F

5′-Agg TgC TAg Agg CCC TgC TA-3′

FAS-R

5′-gTg CAC AgA CAC CTT CCC AT-3′

hyb FAS-X

5′-TAg TgA TgA ggT CCA CAG CAG CAG C-X-3′

hyb FAS-LC

5′-LC-CCC ggT CCT CCA ggC TCT TCA gT-p-3′

Rat-β-actin: NM 031144a, 232-bpb, annealing temperature: 71°C

β-actin-F

5′-CAC ggC ATT gTA ACC AAC Tgg gAC-3′

β-actin-R

5′-CAg Tgg TAC gAC CAg Agg CAT ACA gg-3′

hyb β-actin-X

5′-ACA ggg TgC TCC TCA ggg gCC AC-X-3′

hyb β-actin-LC

5′-LC-CgC AgC TCA TTg TAg AAA gTg Tgg TgC CA-p-3′

  1. aGenBank accession number.
  2. bLength of PCR product (base pairs), -F and -R=forward and reverse primer, hyb=hybridization probe, X=Fluorescein, LC=Light Cycler Red 640, p=phosphate.
Back to article page