Figure 1
From: Rapid detection of VHL exon deletions using real-time quantitative PCR
Selection of optimal primer pairs for Q-PCR analysis of VHL exon 2. Top: melt curve of four different primer sets for VHL exon 2. Primer pair ‘exon 2 set 2’ demonstrated the most consistent melt curves. Bottom: haploid copy numbers for VHL exon 2, and reference genes GPR15 and ZNF80 in calibrator human genomic DNA (commercial mixture of DNA from healthy individuals), a neuroblastoma cell line with a VHL gene deletion (deletion control) and a normal healthy individual (normal control), with three different primer pairs for exon 2 of the VHL gene. The use of ‘exon 2 set 3’ and ‘exon 2 set 4’ results in incorrect copy number quantification. In contrast, the ‘exon 2 set 2’ provides the expected haploid copy numbers.
