Table 1 Q-PCR primer sequences, position, amplicon length and RTPrimerDB ID
From: Rapid detection of VHL exon deletions using real-time quantitative PCR
Gene | Position | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Amplicon length | RTPrimerDB ID |
|---|---|---|---|---|---|
VHL exon 1 | 3p25.3 | CGCCGCATCCACAGCTA | GGCTTCAGACCGTGCTATCG | 78 | 1023 |
VHL exon 2 | 3p25.3 | CAATGTTGACGGACAGCCTATTT | GTCTATCCTGTACTTACCACAACAACCT | 101 | 1024 |
VHL exon 3 | 3p25.3 | GACCTGGAGCGGCTGACA | TACCATCAAAAGCTGAGATGAAACA | 101 | 1025 |
HTR1F | 3p12 | TTCCTGGCATACTCAACAGCAT | CACGTGCTCCATCTTGCATCT | 81 | — |
GPR15 | 3q12.1 | GGTCCCTGGTGGCCTTAATT | TTGCTGGTAATGGGCACACA | 101 | 1022 |
MOX2 | 3q13.2 | CCTCATTGTGACATGGCAGAA | TGTCCTTATAGGCAGGCTGGAT | 101 | — |
ZNF80 | 3q13.31 | CTGTGACCTGCAGCTCATCCT | TAAGTTCTCTGACGTTGACTGATGTG | 120 | 1021 |
