Figure 3

Isolation of the apoptotic IHL component. Flow cytometry contour plots demonstrating the apoptotic IHL component in the cell population isolated using the enzymatic digestion (left) and the enzymatic dispersal (right) protocols. Both protocols isolated approximately similar small proportions of apoptotic cells represented by Annexin-V+ and Annexin-V+PI+ cells with slightly higher proportion for the enzymatic digestion protocol with more dead or necrotic cells (PI+) than the modified protocol. The values inside represent the mean proportion of sorted cells.