Figure 4 | Laboratory Investigation

Figure 4

From: Isolation, purification and flow cytometric analysis of human intrahepatic lymphocytes using an improved technique

Figure 4

Flow cytometry plots demonstrating the differences in CD4+ IHL and CD8+ IHL proportions in the same cell suspension using different purification and cell sorting protocols. (a) Simple filtration protocol and cell sorting (gate) using the traditional light scatter (forward- and side-scatter) parameters (lymphogate-green), and two-colour FACS analysis. (b) Purification protocol using anti-CD2 immunomagnetic beads and cell sorting (gate) using fluorescence gating (anti-CD45-CY5-red) and three-colour FACS analysis. The values outside and inside represent the FACS reading of cell subsets in the studied sample and the mean values of CD4+ and CD8+ cells in all samples, respectively. These plots show that CD4+ and CD8+ IHL populations are underestimated in protocol (a) compared to (b) (P<0.01).

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