Figure 5

Intrahepatic Lymphocyte subsets. Flow cytometry contour plots show the diversity of IHL isolated from histologically normal donor liver specimens. The values inside represent the mean values of sorted subsets. The majority of CD3⁺ IHL express αβTCR (ab) with about one-fifth express γδTCR (gd). CD8⁺ IHL is the predominant population. The donor liver contains IHL populations that express CD4⁺CD8⁺ and CD4⁻CD8⁻ phenotype. Approximately, one-third of IHL express CD56 marker. The donor liver contains large populations of NK cells CD3⁻CD56⁺ IHL and NKT cells (CD3⁺CD56⁺, CD3⁺Vα24⁺ and CD3⁺CD161⁺). The last FACS plot shows the expression of CD161 by CD56⁺ IHL selected using anti-CD56 immunomagnetic beads. The majority of NK and CD3⁺CD56⁺ IHL express CD161 marker.