Figure 5
From: Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma
Repair of DNA SSBs after UV exposure determined by the Comet assay. (a) The representative pattern of cell DNA migration (comet tails) produced by the Comet assay. (b) OPL-7, IB4, OPL-2, Pal-1, and AT(L)5KY cells were exposed to 10 J/m2 of UV, incubated at 37°C for 0–18 h, and assayed as described in Materials and methods. Compared to control cell lines OPL-7 and IB4, OPL-2 and Pal-1 showed failure and inability to repair DNA SSBs after UV exposure. The means (symbol) and standard deviation (error bar) from three experiments is depicted. Symbols represent cell lines: OPL-2 (•), Pal-1 (▪), OPL-7 (○), IB4 (▴), AT(L)5KY (×). (c) Ratio of the DML at 18–6-h incubation after UV exposure, which represented the rate of unrepaired DNA SSBs. Rates in OPL-2 and Pal-1 cells were higher than those in OPL7 and IB4. DML indicates DNA migration length.
